Chemically induced oxidative stress affects ASH neuronal function and behavior in C. elegans.

ABSTRACT: Oxidative stress (OS) impact on a single neuron's function in vivo remains obscure. Using C. elegans as a model organism, we report the effect of paraquat (PQ)-induced OS on wild type worms on the function of the ASH polymodal neuron. By calcium (Ca²⁺) imaging, we quantified ASH activation upon stimulus delivery. PQ-treated worms displayed higher maximum depolarization (peak of the Ca²⁺ transients) compared to untreated animals. PQ had a similar effect on the ASH neuron response time (rising slope of the Ca²⁺ transients), except in very young worms. OS effect on ASH was partially abolished in vitamin C-treated worms. We performed octanol and osmotic avoidance tests, to investigate the OS effect on ASH-dependent behaviors. PQ-treated worms have enhanced avoi

SUBMITTER: Gourgou E 

PROVIDER: S-EPMC5138595 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature