Identification of BnaYUCCA6 as a candidate gene for branch angle in Brassica napus by QTL-seq.

ABSTRACT: Oilseed rape (Brassica napus L.) is one of the most important oil crops in China as well as worldwide. Branch angle as a plant architecture component trait plays an important role for high density planting and yield performance. In this study, bulked segregant analysis (BSA) combined with next generation sequencing technology was used to fine map QTL for branch angle. A major QTL, designated as branch angle 1 (ba1) was identified on A06 and further validated by Indel marker-based classical QTL mapping in an F₂ population. Eighty-two genes were identified in the ba1 region. Among these genes, BnaA0639380D is a homolog of AtYUCCA6. Sequence comparison of BnaA0639380D from small- and big-branch angle oilseed rape lines identified six SNPs and four amino acid variation in the promot

SUBMITTER: Wang H 

PROVIDER: S-EPMC5138835 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature