Targeting B-cell malignancies through human B-cell receptor specific CD4⁺ T cells.

ABSTRACT: The B-cell receptor (BCR) expressed by a clonal B cell tumor is a tumor specific antigen (idiotype). However, the T-cell epitopes within human BCRs which stimulate protective immunity still lack detailed characterization. In this study, we identified 17 BCR peptide-specific CD4⁺ T-cell epitopes derived from BCR heavy and light chain variable region sequences. Detailed analysis revealed these CD4⁺ T-cell epitopes stimulated normal donors' and patients' Th1 CD4⁺ T cells to directly recognize the autologous tumors by secretion of IFNγ, indicating the epitopes are processed and presented by tumor cells. One BCR peptide-specific CD4⁺ T cell line was also cytotoxic and lysed autologous tumor cells through the perforin pathway. Sequence analysis of the

SUBMITTER: Weng J 

PROVIDER: S-EPMC5139634 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature