Synchrotron-based macromolecular crystallography module for an undergraduate biochemistry laboratory course.

ABSTRACT: This paper describes the introduction of synchrotron-based macromolecular crystallography (MX) into an undergraduate laboratory class. An introductory 2 week experimental module on MX, consisting of four laboratory sessions and two classroom lectures, was incorporated into a senior-level biochemistry class focused on a survey of biochemical techniques, including the experimental characterization of proteins. Students purified recombinant protein samples, set up crystallization plates and flash-cooled crystals for shipping to a synchrotron. Students then collected X-ray diffraction data sets from their crystals via the remote interface of the Molecular Biology Consortium beamline (4.2.2) at the Advanced Light Source in Berkeley, CA, USA. Processed diffraction data sets were transferr

SUBMITTER: Stiers KM 

PROVIDER: S-EPMC5140000 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature