The histone deacetylase inhibitor Entinostat enhances polymer-mediated transgene expression in cancer cell lines.

ABSTRACT: Eukaryotic cells maintain an immense amount of genetic information by tightly wrapping their DNA around positively charged histones. While this strategy allows human cells to maintain more than 25,000 genes, histone binding can also block gene expression. Consequently, cells express histone acetyl transferases (HATs) to acetylate histone lysines and release DNA for transcription. Conversely, histone deacetylases (HDACs) are employed for restoring the positive charge on the histones, thereby silencing gene expression by increasing histone-DNA binding. It has previously been shown that histones bind and silence viral DNA, while hyperacetylation of histones via HDAC inhibition restores viral gene expression. In this study, we demonstrate that treatment with Entinostat, an HDAC inhibitor, enha

SUBMITTER: Elmer JJ 

PROVIDER: S-EPMC5140276 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature