Both de novo synthetized and exogenous fatty acids support the growth of hepatocellular carcinoma cells.

ABSTRACT:

Background & aims

Although it is well established that fatty acids (FA) are indispensable for the proliferation and survival of cancer cells in hepatocellular carcinoma (HCC), inhibition of Fatty Acid Synthase (FASN) cannot completely repress HCC cell growth in culture. Thus, we hypothesized that uptake of exogenous FA by cancer cells might play an important role in the development and progression of HCC. Lipoprotein lipase (LPL) is the enzyme that catalyses the hydrolysis of triglycerides into free fatty acids (FFA) and increases the cellular uptake of FA.

Methods

We used immunohistochemistry and quantitative reverse transcription real-time polymerase chain reaction to evaluate LPL expression in human and mouse HCC samples. Using lipoprotein-deficient medium as well as siRN

SUBMITTER: Cao D 

PROVIDER: S-EPMC5140766 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature