Semaphorin 4C: A Novel Component of B-Cell Polarization in Th2-Driven Immune Responses.

ABSTRACT:

Background

Semaphorins are important molecules in embryonic development and multiple semaphorins have been identified as having key roles in immune regulation. To date, there is little known about Semaphorin 4C (Sema4C) in immune biology. We report for the first time that Sema4C is inducible in human and murine B-cells and may be important for normal B-cell development.

Methods

Human tonsillar B-cells were studied following activation via anti-CD40 antibodies in the presence or absence of representative Th1, Th2, and regulatory cytokines. Murine B-cells from WT and Sema4C^-/- mice were similarly stimulated. B-cell phenotyping in WT and Sema4C mutant mice was performed by flow cytometry and lymphoid architecture was studied by immunohistochemistry. Sema4C exp

SUBMITTER: Xue D 

PROVIDER: S-EPMC5141245 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature