Robust Label-free, Quantitative Profiling of Circulating Plasma Microparticle (MP) Associated Proteins.

ABSTRACT: Cells of the vascular system release spherical vesicles, called microparticles, in the size range of 0.1-1 μm induced by a variety of stress factors resulting in variable concentrations between health and disease. Furthermore, microparticles have intercellular communication/signaling properties and interfere with inflammation and coagulation pathways. Today's most used analytical technology for microparticle characterization, flow cytometry, is lacking sensitivity and specificity, which might have led to the publication of contradicting results in the past.We propose the use of nano-liquid chromatography two-stage mass spectrometry as a nonbiased tool for quantitative MP proteome analysis.For this, we developed an improved microparticle isolation protocol and quantified the microparticle p

SUBMITTER: Braga-Lagache S 

PROVIDER: S-EPMC5141277 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature