A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles.

ABSTRACT: A novel detection method of small molecules, competitive bio-barcode amplification immunoassay, was developed and described in this report. Through the gold nanoparticles (AuNPs) probe and magnetic nanoparticles (MNPs) probe we prepared, only one monoclonal antibody can be used to detect small molecules. The competitive bio-barcode amplification immunoassay overcomes the obstacle that the bio-barcode assay cannot be used in small molecular detection, as two antibodies are unable to combine to one small molecule due to its small molecular structure. The small molecular compounds, triazophos, were selected as targets for the competitive bio-barcode amplification immunoassay. The linear range of detection was from 0.04 ng mL^-1 to 10 ng mL^-1, and the limit of detection (L

SUBMITTER: Du P 

PROVIDER: S-EPMC5141433 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature