Size Specific Transfection to Mammalian Cells by Micropillar Array Electroporation.

ABSTRACT: Electroporation serves as a promising non-viral gene delivery approach, while its current configuration carries several drawbacks associated with high-voltage electrical pulses and heterogeneous treatment on individual cells. Here we developed a new micropillar array electroporation (MAE) platform to advance the electroporation-based delivery of DNA and RNA probes into mammalian cells. By introducing well-patterned micropillar array texture on the electrode surface, the number of pillars each cell faces varies with its plasma membrane surface area, despite their large population and random locations. In this way, cell size specific electroporation is conveniently carried out, contributing to a 2.5~3 fold increase on plasmid DNA transfection and an additional 10-55% transgene knockdown with

SUBMITTER: Zu Y 

PROVIDER: S-EPMC5141490 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature