Antibodies to inositol 1,4,5-triphosphate receptor 1 in patients with cerebellar disease.

ABSTRACT:

Objective

To describe newly identified autoantibodies associated with cerebellar disorders.

Design/methods

We first screened the sera of 15 patients with cerebellar ataxia, without any known associated autoantibodies, with immunocytochemistry on mouse brain. After characterization and validation of a newly identified antibody, 85 additional patients with suspected autoimmune cerebellar disease were screened using a cell-based assay.

Results

Immunoglobulin G from one of the first 15 patients demonstrated a distinct staining pattern on Purkinje neurons. This autoantibody, as characterized further by immunoprecipitation and mass spectrometry, was binding inositol 1,4,5-triphosphate receptor 1 (IP3R1), an intracellular channel that mediates the release of Ca²⁺

SUBMITTER: Fouka P 

PROVIDER: S-EPMC5141524 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature