Neurochondrin is a neuronal target antigen in autoimmune cerebellar degeneration.

ABSTRACT:

Objective

To report on a novel neuronal target antigen in 3 patients with autoimmune cerebellar degeneration.

Methods

Three patients with subacute to chronic cerebellar ataxia and controls underwent detailed clinical and neuropsychological assessment together with quantitative high-resolution structural MRI. Sera and CSF were subjected to comprehensive autoantibody screening by indirect immunofluorescence assay (IFA) and immunoblot. Immunoprecipitation with lysates of hippocampus and cerebellum combined with mass spectrometric analysis was used to identify the autoantigen, which was verified by recombinant expression in HEK293 cells and use in several immunoassays. Multiparameter flow cytometry was performed on peripheral blood and CSF, and peripheral blood was subjected to

SUBMITTER: Miske R 

PROVIDER: S-EPMC5141526 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature