Microbial production of next-generation stevia sweeteners.

ABSTRACT:

Background

The glucosyltransferase UGT76G1 from Stevia rebaudiana is a chameleon enzyme in the targeted biosynthesis of the next-generation premium stevia sweeteners, rebaudioside D (Reb D) and rebaudioside M (Reb M). These steviol glucosides carry five and six glucose units, respectively, and have low sweetness thresholds, high maximum sweet intensities and exhibit a greatly reduced lingering bitter taste compared to stevioside and rebaudioside A, the most abundant steviol glucosides in the leaves of Stevia rebaudiana.

Results

In the metabolic glycosylation grid leading to production of Reb D and Reb M, UGT76G1 was found to catalyze eight different reactions all involving 1,3-glucosylation of steviol C ₁₃- and C ₁₉-bound glucoses. Four of these reactio

SUBMITTER: Olsson K 

PROVIDER: S-EPMC5142139 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature