1.92 Angstrom Zinc-Free APOBEC3F Catalytic Domain Crystal Structure.

ABSTRACT: The APOBEC3 family of DNA cytosine deaminases is capable of restricting the replication of HIV-1 and other pathogens. Here, we report a 1.92 Å resolution crystal structure of the Vif-binding and catalytic domain of APOBEC3F (A3F). This structure is distinct from the previously published APOBEC and phylogenetically related deaminase structures, as it is the first without zinc in the active site. We determined an additional structure containing zinc in the same crystal form that allows direct comparison with the zinc-free structure. In the absence of zinc, the conserved active site residues that normally participate in zinc coordination show unique conformations, including a 90 degree rotation of His249 and disulfide bond formation between Cys280 and Cys283. We found that zinc coordination i

SUBMITTER: Shaban NM 

PROVIDER: S-EPMC5142242 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature