Phenotypic and molecular detection of the bla _KPC gene in clinical isolates from inpatients at hospitals in Sao Luis, MA, Brazil.

ABSTRACT:

Background

Bacteria that produce Klebsiella pneumoniae carbapenemases (KPCs) are resistant to broad-spectrum β-lactam antibiotics. The objective of this study was to phenotypically and genotypically characterize the antibiotic susceptibility to carbapenems of 297 isolates recovered from clinical samples obtained from inpatients at 16 hospitals in São Luis (Maranhão, Brazil).

Methods

The study was conducted using phenotypic tests and molecular methods, including polymerase chain reaction (PCR), sequencing and enterobacterial repetitive intergenic consensus (ERIC)-PCR. The nonparametric chi-square test of independence was used to evaluate the associations between the bacterial bla _KPC gene and the modified Hodge test, and the chi-square adherence test was used to as

SUBMITTER: Ribeiro PC 

PROVIDER: S-EPMC5142414 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature