The role of nucleotide composition in premature termination codon recognition.

ABSTRACT:

Background

It is not fully understood how a termination codon is recognized as premature (PTC) by the nonsense-mediated decay (NMD) machinery. This is particularly true for transcripts lacking an exon junction complex (EJC) along their 3' untranslated region (3'UTR), and thus degrade through the EJC-independent NMD pathway.

Results

Here, we analyzed data of transcript stability change following NMD repression and identified over 200 EJC-independent NMD-targets. We examined many features characterizing these transcripts, and compared them to NMD-insensitive transcripts, as well as to a group of transcripts that are destabilized following NMD repression (destabilized transcripts).

Conclusions

We found that none of the known NMD-triggering features, such as the presence

SUBMITTER: Zahdeh F 

PROVIDER: S-EPMC5142417 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature