Role of BCL2-associated athanogene 1 in differential sensitivity of human endothelial cells to glucocorticoids.

ABSTRACT:

Objective

Chronic therapy with synthetic glucocorticoids has been associated with cardiovascular side effects, although differential interindividual susceptibility to glucocorticoids has been observed. The objective of this study was to identify the molecular mechanisms leading to differential glucocorticoid responses in endothelial cells.

Approach and results

We tested the sensitivity of 42 human umbilical vein endothelial cells (HUVECs) to dexamethasone as determined by changes in gene expression, promoter transactivation, and procoagulant activity. We identified that 16 HUVECs were sensitive in every test, 14 HUVECs were sensitive in at least 1 test and 12 HUVECs were resistant in every test to dexamethasone. Nuclear translocation assays revealed that Dex-sensitive HUVECs

SUBMITTER: Mata-Greenwood E 

PROVIDER: S-EPMC5142448 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature