Robust generation of transgenic mice by simple hypotonic solution mediated delivery of transgene in testicular germ cells.

ABSTRACT: Our ability to decipher gene sequences has increased enormously with the advent of modern sequencing tools, but the ability to divulge functions of new genes have not increased correspondingly. This has caused a remarkable delay in functional interpretation of several newly found genes in tissue and age specific manner, limiting the pace of biological research. This is mainly due to lack of advancements in methodological tools for transgenesis. Predominantly practiced method of transgenesis by pronuclear DNA-microinjection is time consuming, tedious, and requires highly skilled persons for embryo-manipulation. Testicular electroporation mediated transgenesis requires use of electric current to testis. To this end, we have now developed an innovative technique for making transgenic mice by

SUBMITTER: Usmani A 

PROVIDER: S-EPMC5142465 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature