Optimization of techniques for multiple platform testing in small, precious samples such as human chorionic villus sampling.

ABSTRACT:

Background

Multiple testing to understand global changes in gene expression based on genetic and epigenetic modifications is evolving. Chorionic villi, obtained for prenatal testing, is limited, but can be used to understand ongoing human pregnancies. However, optimal storage, processing and utilization of CVS for multiple platform testing have not been established.

Results

Leftover CVS samples were flash-frozen or preserved in RNAlater. Modifications to standard isolation kits were performed to isolate quality DNA and RNA from samples as small as 2-5 mg. RNAlater samples had significantly higher RNA yields and quality and were successfully used in microarray and RNA-sequencing (RNA-seq). RNA-seq libraries generated using 200 versus 800-ng RNA showed similar biological coeff

SUBMITTER: Pisarska MD 

PROVIDER: S-EPMC5142835 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature