Crystal structures of the TRIC trimeric intracellular cation channel orthologues.

ABSTRACT: Ca²⁺ release from the sarcoplasmic reticulum (SR) and endoplasmic reticulum (ER) is crucial for muscle contraction, cell growth, apoptosis, learning and memory. The trimeric intracellular cation (TRIC) channels were recently identified as cation channels balancing the SR and ER membrane potentials, and are implicated in Ca²⁺ signaling and homeostasis. Here we present the crystal structures of prokaryotic TRIC channels in the closed state and structure-based functional analyses of prokaryotic and eukaryotic TRIC channels. Each trimer subunit consists of seven transmembrane (TM) helices with two inverted repeated regions. The electrophysiological, biochemical and biophysical analyses revealed that TRIC channels possess an ion-conducting pore within each subunit, and tha

SUBMITTER: Kasuya G 

PROVIDER: S-EPMC5143425 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature