Mitochondrial co-chaperone protein Tid1 is required for energy homeostasis during skeletal myogenesis.

ABSTRACT:

Background

Tid1 is a mitochondrial co-chaperone protein and its transcript is abundantly expressed in skeletal muscle tissues. However, the physiological function of Tid1 during skeletal myogenesis remains unclear.

Methods

In vitro induced differentiation assay of mouse myoblast C2C12 cells was applied to examine the physiological role of Tid1 during skeletal myogenesis. In addition, transgenic mice with muscle specific (HSA-Cre) Tid1 deletion were established and examined to determine the physiological function of Tid1 during skeletal muscle development in vivo.

Results

Expression of Tid1 protein was upregulated in the differentiated C2C12 cells, and the HSA-Tid1^f/f mice displayed muscular dystrophic phenotype. The expression of myosin heavy chain (MyHC),

SUBMITTER: Cheng LH 

PROVIDER: S-EPMC5143475 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature