Induction and characterization of taxol-resistance phenotypes with a transiently expressed artificial transcriptional activator library.

ABSTRACT: Phenotype-based functional genomic methods are useful for the identification of genes that are related to a particular biological function or disease. Essential to this approach is the ability to regulate the expression of selected genes. Artificial transcription factors (ATFs) are key molecular tools that selectively regulate gene expression in vivo. Here, we use an ATF library to identify genes that participate in rendering a cell resistant to the drug Taxol, a potent anti-cancer drug that binds to tubulin and inhibits cell division. The library, which encodes ATFs that activate (rather than inhibit) transcription, was introduced into a HeLa cell line, and Taxol-resistant cells were selected. After eight rounds of selection, we identified two ATFs that significantly increased the level o

SUBMITTER: Lee DK 

PROVIDER: S-EPMC514398 | biostudies-literature | 2004 Aug

REPOSITORIES: biostudies-literature