Subchronic perfluorooctanesulfonate (PFOS) exposure induces elevated mutant frequency in an in vivo λ transgenic medaka mutation assay.

ABSTRACT: Perfluorooctanesulfonate (PFOS) has been widely detected in the environment, wildlife and humans, but few studies have ever examined its mutagenic effect in vivo. In the present study, we use a transgenic fish model, the λ transgenic medaka, to evaluate the potential mutagenicity of PFOS in vivo following a subchronic exposure of 30 days. The mutant frequency of cII target gene was 3.46 × 10^-5 in liver tissue from control fish, which increased by 1.4-fold to 4.86 × 10^-5 in fish exposed to 6.7 μg/L PFOS, 1.55-fold to 5.36 × 10^-5 in fish exposed to 27.6 μg/L PFOS, and 2.02-fold to 6.99 × 10^-5 in fish exposed to 87.6 μg/L PFOS. This dose-dependent increase of mutant frequency was also accompanied with mutational spectrum changes associated with PFOS

SUBMITTER: Chen Y 

PROVIDER: S-EPMC5144067 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature