Real time optical Biopsy: Time-resolved Fluorescence Spectroscopy instrumentation and validation.

ABSTRACT: The Time-resolved fluorescence spectroscopy (TR-FS) has the potential to differentiate tumor and normal tissue in real time during surgical excision. In this manuscript, we describe the design of a novel TR-FS device, along with preliminary data on detection accuracy for fluorophores in a mixture. The instrument is capable of near real-time fluorescence lifetime acquisition in multiple spectral bands and analysis. It is also able to recover fluorescence lifetime with sub-20ps accuracy as validated with individual organic fluorescence dyes and dye mixtures yielding lifetime values for standard fluorescence dyes that closely match with published data. We also show that TR-FS is able to quantify the relative concentration of fluorescence dyes in a mixture by the unmixing of lifetime decays. W

SUBMITTER: Kittle DS 

PROVIDER: S-EPMC5144092 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature