Methionine sulfoxides in serum proteins as potential clinical biomarkers of oxidative stress.

ABSTRACT: Oxidative stress contributes to the pathophysiology of a variety of diseases, and circulating biomarkers of its severity remains a topic of great interest for researchers. Our peptidomic strategy enables accurate and reproducible analysis of circulating proteins/peptides with or without post-translational modifications. Conventional wisdom holds that hydrophobic methionines exposed to an aqueous environment or experimental handling procedures are vulnerable to oxidation. However, we show that the mass spectra intensity ratio of oxidized to non-oxidized methionine residues in serum tryptic proteins can be accurately quantified using a single drop of human serum and give stable and reproducible results. Our data demonstrate that two methionine residues in serum albumin (Met-111 and Met-147)

SUBMITTER: Suzuki S 

PROVIDER: S-EPMC5144094 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature