Robust Transgene Expression from Bicistronic mRNA in the Green Alga Chlamydomonas reinhardtii.

ABSTRACT: The unicellular green alga Chlamydomonas reinhardtii is a model organism that provides an opportunity to understand the evolution and functional biology of the lineage that includes the land plants, as well as aspects of the fundamental core biology conserved throughout the eukaryotic phylogeny. Although many tools are available to facilitate genetic, molecular biological, biochemical, and cell biological studies in Chlamydomonas, expression of unselected transgenes of interest (GOIs) has been challenging. In most methods used previously, the GOI and a selectable marker are expressed from two separate mRNAs, so that their concomitant expression is not guaranteed. In this study, we developed constructs that allow expression of an upstream GOI and downstream selectable marker from a single b

SUBMITTER: Onishi M 

PROVIDER: S-EPMC5144980 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature