Outside-in integrin signalling regulates haematopoietic stem cell function via Periostin-Itgav axis.

ABSTRACT: Integrins play an important role in haematopoietic stem cell (HSC) maintenance in the bone marrow niche. Here, we demonstrate that Periostin (Postn) via interaction with Integrin-αv (Itgav) regulates HSC proliferation. Systemic deletion of Postn results in peripheral blood (PB) anaemia, myelomonocytosis and lymphopenia, while the number of phenotypic HSCs increases in the bone marrow. Postn^-/- mice recover faster from radiation injury with concomitant loss of primitive HSCs. HSCs from Postn^-/- mice show accumulation of DNA damage generally associated with aged HSCs. Itgav deletion in the haematopoietic system leads to a similar PB phenotype and HSC-intrinsic repopulation defects. Unaffected by Postn, Vav-Itgav^-/- HSCs proliferate faster in vitro, illustrati

SUBMITTER: Khurana S 

PROVIDER: S-EPMC5146274 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature