L-type calcium channels regulate filopodia stability and cancer cell invasion downstream of integrin signalling.

ABSTRACT: Mounting in vitro, in vivo and clinical evidence suggest an important role for filopodia in driving cancer cell invasion. Using a high-throughput microscopic-based drug screen, we identify FDA-approved calcium channel blockers (CCBs) as potent inhibitors of filopodia formation in cancer cells. Unexpectedly, we discover that L-type calcium channels are functional and frequently expressed in cancer cells suggesting a previously unappreciated role for these channels during tumorigenesis. We further demonstrate that, at filopodia, L-type calcium channels are activated by integrin inside-out signalling, integrin activation and Src. Moreover, L-type calcium channels promote filopodia stability and maturation into talin-rich adhesions through the spatially restricted regulation of calcium entry a

SUBMITTER: Jacquemet G 

PROVIDER: S-EPMC5146291 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature