Skeletal muscle characteristics are preserved in hTERT/cdk4 human myogenic cell lines.

ABSTRACT:

Background

hTERT/cdk4 immortalized myogenic human cell lines represent an important tool for skeletal muscle research, being used as therapeutically pertinent models of various neuromuscular disorders and in numerous fundamental studies of muscle cell function. However, the cell cycle is linked to other cellular processes such as integrin regulation, the PI3K/Akt pathway, and microtubule stability, raising the question as to whether genetic modification related to the cell cycle results in secondary effects that could undermine the validity of these cell models.

Results

Here we subjected five healthy and disease muscle cell isolates to transcriptomic analysis, comparing immortalized lines with their parent primary populations in both differentiated and undifferentiated state

SUBMITTER: Thorley M 

PROVIDER: S-EPMC5146814 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature