Epigenetics of amphetamine-induced sensitization: HDAC5 expression and microRNA in neural remodeling.

ABSTRACT:

Background

Histone deacetylase (HDAC) activities modify chromatin structure and play a role in learning and memory during developmental processes. Studies of adult mice suggest HDACs are involved in neural network remodeling in brain repair, but its function in drug addiction is less understood. We aimed to examine in vivo HDAC5 expression in a preclinical model of amphetamine-induced sensitization (AIS) of behavior. We generated specific contrast agents to measure HDAC5 levels by in vivo molecular contrast-enhanced (MCE) magnetic resonance imaging (MRI) in amphetamine-naïve mice as well as in mice with AIS. To validate the MRI results we used ex vivo methods including in situ hybridization, RT-PCR, immunohistochemistry, and transmision electron microscopy.

Methods

We compar

SUBMITTER: Liu PK 

PROVIDER: S-EPMC5146867 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature