Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors.

ABSTRACT:

Background

Polycistronic retroviral vectors that contain several therapeutic genes linked via internal ribosome entry sites (IRES), provide new and effective tools for the co-expression of exogenous cDNAs in clinical gene therapy protocols. For example, tricistronic retroviral vectors could be used to genetically modify antigen presenting cells, enabling them to express different co-stimulatory molecules known to enhance tumor cell immunogenicity.

Results

We have constructed and compared different retroviral vectors containing two co-stimulatory molecules (CD70, CD80) and selectable marker genes linked to different IRES sequences (IRES from EMCV, c-myc, FGF-2 and HTLV-1). The tricistronic recombinant amphotropic viruses containing the IRES from EMCV, FGF-2 or HTLV-1 were equ

SUBMITTER: Douin V 

PROVIDER: S-EPMC514710 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature