Encapsulation of eugenyl acetate in PHBV using SEDS technique and in vitro release evaluation.

ABSTRACT: Eugenyl acetate obtained via enzymatic esterification using Lipozyme TL IM enzyme was encapsulated in biopolymer poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV) through solution-enhanced dispersion by supercritical fluids (SEDS). Produced particles were characterized by SEM and confocal microscopy techniques and in addition in vitro release assays were performed in isopropanol and ethyl acetate. Experimental micronization conditions comprised 8 and 10 MPa, 308 and 313 K and eugenyl acetate concentration ranging from 5 to 20 mg mL^-1, keeping PHBV concentration constant (20 mg mL^-1 in dichloromethane). The maximum encapsulation efficiency was 58.0 % for 5 mg mL^-1of eugenyl acetate at 8 MPa and 308 K. The morphology of the encapsulated particles for most of

SUBMITTER: Loss RA 

PROVIDER: S-EPMC5147702 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature