Combgap Promotes Ovarian Niche Development and Chromatin Association of EcR-Binding Regions in BR-C.

ABSTRACT: The development of niches for tissue-specific stem cells is an important aspect of stem cell biology. Determination of niche size and niche numbers during organogenesis involves precise control of gene expression. How this is achieved in the context of a complex chromatin landscape is largely unknown. Here we show that the nuclear protein Combgap (Cg) supports correct ovarian niche formation in Drosophila by controlling ecdysone-Receptor (EcR)- mediated transcription and long-range chromatin contacts in the broad locus (BR-C). Both cg and BR-C promote ovarian growth and the development of niches for germ line stem cells. BR-C levels were lower when Combgap was either reduced or over-expressed, indicating an intricate regulation of the BR-C locus by Combgap. Polytene chromosome stains showe

SUBMITTER: Hitrik A 

PROVIDER: S-EPMC5147775 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature