A Cost-Effective Method to Assemble Biomimetic 3D Cell Culture Platforms.

ABSTRACT:

Methods

We utilized the hAM to provide the biological and the three dimensional (3D) topographic components of the prototype. The 3D nano-roughness of the hAM was characterized using surface electron microscopy and surface image analysis (ImageJ and SurfaceJ). We developed additional macro-scale and micro-scale versions of the platform which provided additional shear stress factors to simulate the fluid dynamics of the in vivo extracellular fluids.

Results

Three models of varying complexities of the prototype were assembled. A well-defined 3D surface modulation of the hAM in comparable to commercial 3D biomaterial culture substrates was achieved without complex fabrication and with significantly lower cost. Performance of the prototype was demonstrated through culture of pri

SUBMITTER: Khalil S 

PROVIDER: S-EPMC5147837 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature