Cell culture-based profiling across mammals reveals DNA repair and metabolism as determinants of species longevity.

ABSTRACT: Mammalian lifespan differs by >100 fold, but the mechanisms associated with such longevity differences are not understood. Here, we conducted a study on primary skin fibroblasts isolated from 16 species of mammals and maintained under identical cell culture conditions. We developed a pipeline for obtaining species-specific ortholog sequences, profiled gene expression by RNA-seq and small molecules by metabolite profiling, and identified genes and metabolites correlating with species longevity. Cells from longer lived species up-regulated genes involved in DNA repair and glucose metabolism, down-regulated proteolysis and protein transport, and showed high levels of amino acids but low levels of lysophosphatidylcholine and lysophosphatidylethanolamine. The amino acid patterns were recapitula

SUBMITTER: Ma S 

PROVIDER: S-EPMC5148604 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature