Structural Aspects of Heparan Sulfate Binding to Robo1-Ig1-2.

ABSTRACT: Roundabout 1, or Robo1, is a cell surface signaling molecule important in axon guidance. Its interaction with heparan sulfate (HS) and members of the Slit protein family is essential to its activity, making characterization of these interactions by structural methods, such as NMR, highly desirable. However, the fact that Robo1 is a glycosylated protein prevents employment of commonly used bacterial hosts for expression of properly glycosylated forms with the uniform ¹⁵N, ¹³C, and ²H labeling needed for NMR studies. Here, we apply an alternative methodology, based on labeling with a single amino acid type and high structural content NMR data, to characterize a two-domain construct of glycosylated Robo1 (Robo1-Ig1-2) interacting with a synthetic HS tetramer (

SUBMITTER: Gao Q 

PROVIDER: S-EPMC5148660 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature