High-Throughput Indirect Quantitation of ¹³C Enriched Metabolites Using ¹H NMR.

ABSTRACT: Nuclear magnetic resonance (NMR) spectroscopy is widely used in metabolomics to perform quantitative profiling of low-molecular weight compounds from biological specimens. The measurement of endogenous metabolites using NMR has proven to be a powerful tool to identify new metabolic biomarkers in physiological and pathological conditions, and to study and evaluate treatment efficiency. In this study we present a rapid approach to indirectly quantify ¹³C enriched molecules using one-dimensional (1D) ¹H NMR. We demonstrate this approach using isotopically labeled [1,6-¹³C]glucose and in four different cell lines. We confirm the applicability of this approach for treatment follow-up, utilizing a renal cancer cell line with rapamycin as a tool compound to study

SUBMITTER: Di Gialleonardo V 

PROVIDER: S-EPMC5148664 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature