Validation of internal reference genes for relative quantitation studies of gene expression in human laryngeal cancer.

ABSTRACT:

Background

The aim of this study was to determine the expression stabilities of 12 common internal reference genes for the relative quantitation analysis of target gene expression performed by reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) in human laryngeal cancer.

Methods

Hep-2 cells and 14 laryngeal cancer tissue samples were investigated. The expression characteristics of 12 internal reference gene candidates (18S rRNA, GAPDH, ACTB, HPRT1, RPL29, HMBS, PPIA, ALAS1, TBP, PUM1, GUSB, and B2M) were assessed by RT-qPCR. The data were analyzed by three commonly used software programs: geNorm, NormFinder, and BestKeeper.

Results

The use of the combination of four internal reference genes was more appropriate than the use of a single int

SUBMITTER: Wang X 

PROVIDER: S-EPMC5149058 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature