Murine supraspinatus tendon injury model to identify the cellular origins of rotator cuff healing.

ABSTRACT: Purpose of this study: To elucidate the origin of cell populations that contribute to rotator cuff healing, we developed a mouse surgical model where a full-thickness, central detachment is created in the supraspinatus.

Materials and methods

Three different inducible Cre transgenic mice with Ai9-tdTomato reporter expression (PRG4-9, αSMA-9, and AGC-9) were used to label different cell populations in the shoulder. The defect was created surgically in the supraspinatus. The mice were injected with tamoxifen at surgery to label the cells and sacrificed at 1, 2, and 5 weeks postoperatively. Frozen sections were fluorescently imaged then stained with Toluidine Blue and re-imaged.

Results

Three notable changes were apparent postoperatively. (1) A long thin layer of tissue formed on

SUBMITTER: Yoshida R 

PROVIDER: S-EPMC5149426 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature