Epicutaneous immunotherapy induces gastrointestinal LAP⁺ regulatory T cells and prevents food-induced anaphylaxis.

ABSTRACT:

Background

The attempt to induce oral tolerance as a treatment for food allergy has been hampered by a lack of sustained clinical protection. Immunotherapy by nonoral routes, such as the skin, may be more effective for the development of maintained tolerance to food allergens.

Objective

We sought to determine the efficacy and mechanism of tolerance induced by epicutaneous immunotherapy (EPIT) in a model of food-induced anaphylaxis.

Methods

C3H/HeJ mice were sensitized to ovalbumin (OVA) orally or through the skin and treated with EPIT using OVA-Viaskin patches or oral immunotherapy using OVA. Mice were orally challenged with OVA to induce anaphylaxis. Antigen-specific regulatory T (Treg)-cell induction was assessed by flow cytometry using a transgenic T-cell transfer

SUBMITTER: Tordesillas L 

PROVIDER: S-EPMC5149454 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature