Four-dimensional live imaging of hemodynamics in mammalian embryonic heart with Doppler optical coherence tomography.

ABSTRACT: Hemodynamic analysis of the mouse embryonic heart is essential for understanding the functional aspects of early cardiogenesis and advancing the research in congenital heart defects. However, high-resolution imaging of cardiac hemodynamics in mammalian models remains challenging, primarily due to the dynamic nature and deep location of the embryonic heart. Here we report four-dimensional micro-scale imaging of blood flow in the early mouse embryonic heart, enabling time-resolved measurement and analysis of flow velocity throughout the heart tube. Our method uses Doppler optical coherence tomography in live mouse embryo culture, and employs a post-processing synchronization approach to reconstruct three-dimensional data over time at a 100 Hz volume rate. Experiments were performed on live m

SUBMITTER: Wang S 

PROVIDER: S-EPMC5152918 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature