Biochemical Characterization, Thermal Stability, and Partial Sequence of a Novel Exo-Polygalacturonase from the Thermophilic Fungus Rhizomucor pusillus A13.36 Obtained by Submerged Cultivation.

ABSTRACT: This work reports the production of an exo-polygalacturonase (exo-PG) by Rhizomucor pusillus A13.36 in submerged cultivation (SmC) in a shaker at 45°C for 96 h. A single pectinase was found and purified in order to analyze its thermal stability, by salt precipitation and hydrophobic interaction chromatography. The pectinase has an estimated Mw of approximately 43.5-47 kDa and optimum pH of 4.0 but is stable in pH ranging from 3.5 to 9.5 and has an optimum temperature of 61°C. It presents thermal stability between 30 and 60°C, has 70% activation in the presence of Ca²⁺, and was tested using citrus pectin with a degree of methyl esterification (DE) of 26%. E_a(d) for irreversible denaturation was 125.5 kJ/mol with positive variations of entrop

SUBMITTER: Trindade LV 

PROVIDER: S-EPMC5153499 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature