Functional proteomics-aided selection of protease inhibitors for herbivore insect control.

ABSTRACT: Studies have reported the potential of protease inhibitors to engineer insect resistance in transgenic plants but the general usefulness of this approach in crop protection still remains to be established. Insects have evolved strategies to cope with dietary protease inhibitors, such as the use of proteases recalcitrant to inhibition, that often make the selection of effective inhibitors very challenging. Here, we used a functional proteomics approach for the 'capture' of Cys protease targets in crude protein extracts as a tool to identify promising cystatins for plant improvement. Two cystatins found to differ in their efficiency to capture Cys proteases of the coleopteran pest Leptinotarsa decemlineata also differed in their usefulness to produce transgenic potato lines resistant to this

SUBMITTER: Rasoolizadeh A 

PROVIDER: S-EPMC5153846 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature