Non-coding single nucleotide variants affecting estrogen receptor binding and activity.

ABSTRACT:

Background

Estrogen receptor (ER) activity is critical for the development and progression of the majority of breast cancers. It is known that ER is differentially bound to DNA leading to transcriptomic and phenotypic changes in different breast cancer models. We investigated whether single nucleotide variants (SNVs) in ER binding sites (regSNVs) contribute to ER action through changes in the ER cistrome, thereby affecting disease progression. Here we developed a computational pipeline to identify SNVs in ER binding sites using chromatin immunoprecipitation sequencing (ChIP-seq) data from ER+ breast cancer models.

Methods

ER ChIP-seq data were downloaded from the Gene Expression Omnibus (GEO). GATK pipeline was used to identify SNVs and the MACS algorithm was employed to cal

SUBMITTER: Bahreini A 

PROVIDER: S-EPMC5154163 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature