Unusual Five Copies and Dual Forms of nrdB in "Candidatus Liberibacter asiaticus": Biological Implications and PCR Detection Application.

ABSTRACT: "Candidatus Liberibacter asiaticus" (CLas), a non-culturable α-proteobacterium, is associated with citrus Huanglongbing (HLB, yellow shoot disease) currently threatening citrus production worldwide. Here, the whole genome sequence of CLas strain A4 from Guangdong of China was analyzed. Five copies of nrdB, encoding β-subunit of ribonucleotide reductase (RNR), a critical enzyme involving bacterial proliferation, were found. Three nrdB copies were in long form (nrdB^L, 1,059 bp) and two were in short form (nrdB^S, 378 bp). nrdB^S shared >99% identity to 3' end of nrdB^L and had no active site. Sequences of CLas nrdB genes formed a distinct monophyletic lineage among eubacteria. To make use of the high copy number feature, a nrdB-based primer set RNRf/R

SUBMITTER: Zheng Z 

PROVIDER: S-EPMC5154197 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature