Imaging of radicals following injury or acute stress in peripheral nerves with activatable fluorescent probes.

ABSTRACT: Peripheral nerve injury evokes a complex cascade of chemical reactions including generation of molecular radicals. Conversely, the reactions within nerve induced by stress are difficult to directly detect or measure to establish causality. Monitoring these reactions in vivo would enable deeper understanding of the nature of the injury and healing processes. Here, we utilized near-infrared fluorescence molecular probes delivered via intra-neural injection technique to enable live, in vivo imaging of tissue response associated with nerve injury and stress. These initially quenched fluorescent probes featured specific sensitivity to hydroxyl radicals and become fluorescent upon encountering reactive oxygen species (ROS). Intraneurally delivered probes demonstrated rapid activation in injured

SUBMITTER: Zhou H 

PROVIDER: S-EPMC5154790 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature