RAB43 facilitates cross-presentation of cell-associated antigens by CD8α+ dendritic cells.

ABSTRACT: In this study, to examine cross-presentation by classical dendritic cells (DCs; cDCs), we evaluated the role of RAB43, a protein found to be selectively expressed by Batf3-dependent CD8α⁺ and CD103⁺ compared with other DC subsets and immune lineages. Using a specific monoclonal antibody, we localized RAB43 expression to the Golgi apparatus and LAMP1^- cytoplasmic vesicles. Mice with germline or conditional deletion of Rab43 are viable and fertile and have normal development of cDCs but show a defect for in vivo and in vitro cross-presentation of cell-associated antigen. This defect is specific to cDCs, as Rab43-deficient monocyte-derived DCs showed no defect in cross-presentation of cell-associated antigen. These results suggest that RAB43 provides a special

SUBMITTER: Kretzer NM 

PROVIDER: S-EPMC5154939 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature