Genomewide Dam Methylation in Escherichia coli during Long-Term Stationary Phase.

ABSTRACT: DNA methylation in prokaryotes is widespread. The most common modification of the genome is the methylation of adenine at the N-6 position. In Escherichia coli K-12 and many gammaproteobacteria, this modification is catalyzed by DNA adenine methyltransferase (Dam) at the GATC consensus sequence and is known to modulate cellular processes including transcriptional regulation of gene expression, initiation of chromosomal replication, and DNA mismatch repair. While studies thus far have focused on the motifs associated with methylated adenine (meA), the frequency of meA across the genome, and temporal dynamics during early periods of incubation, here we conduct the first study on the temporal dynamics of adenine methylation in E. coli by Dam throughout all five phases of the bac

SUBMITTER: Westphal LL 

PROVIDER: S-EPMC5155068 | biostudies-literature | 2016 Nov-Dec

REPOSITORIES: biostudies-literature