Transcriptional analysis of degenerate strain Clostridium beijerinckii DG-8052 reveals a pleiotropic response to CaCO₃-associated recovery of solvent production.

ABSTRACT: Degenerate Clostridium beijerinckii strain (DG-8052) can be partially recovered by supplementing CaCO₃ to fermentation media. Genome resequencing of DG-8052 showed no general regulator mutated. This study focused on transcriptional analysis of DG-8052 and its response to CaCO₃ treatment via microarray. The expressions of 5168 genes capturing 98.6% of C. beijerinckii NCIMB 8052 genome were examed. The results revealed that with addition of CaCO₃ 565 and 916 genes were significantly up-regulated, and 704 and 1044 genes significantly down-regulated at acidogenic and solventogenic phase of DG-8052, respectively. These genes are primarily responsible for glycolysis to solvent/acid production (poR, pfo), solventogensis (buk, ctf, aldh, adh, bcd) and sporulation (

SUBMITTER: Jiao S 

PROVIDER: S-EPMC5155275 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature