Expression of somatostatin receptors 2 and 5 in circulating tumour cells from patients with neuroendocrine tumours.

ABSTRACT:

Background

Neuroendocrine tumours (NET) overexpress somatostatin receptors (SSTR) that can be targeted for therapy. Somatostatin receptor expression is routinely measured by molecular imaging but the resolution is insufficient to define heterogeneity. We hypothesised that SSTR expression could be measured on circulating tumour cells (CTCs) and used to investigate heterogeneity of expression and track changes during therapy.

Methods

MCF-7 cells were transfected with SSTR2 or 5 and spiked into donor blood for analysis by CellSearch. Optimum anti-SSTR antibody concentration and exposure time were determined, and flow cytometry was used to evaluate assay sensitivity. For clinical evaluation, blood was analysed by CellSearch, and SSTR2/5 immunohistochemistry was performed on matc

SUBMITTER: Childs A 

PROVIDER: S-EPMC5155369 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature